16
Samples were considered including hand-washing children
(part breastfeeding and children exclusively breastfed
children) and their mothers (partially breastfed, mother
exclusively breastfeeding mother), food dishes, the remnants
food and drinking water. The overall level of isolation of Escherichia coli, S. dysentery, Salmonella sp. , Enterobacter sp. ,
K. pneumonia, and Serratia marcescens from the collected samples
turned 38. 01, 23. 64, 18. 84, 9. 26, 6. 70 >> << and strattera 3. 51%, respectively (Table 3). The highest isolation rate
E. coli, S. dysentery and Samonella sp. ,
Enterobacter sp. K. pneumonia, S. marcescens
were seen in the remains of a meal, PBM, EBC and their mother
EBC, and feeding dishes, respectively. In >> << On the other hand, the low rate of selection of Escherichia coli, S. dysentery
, and Samonella sp. observed in FA, DW, LF,
, respectively, while the Enterobacter sp. K. pneumonia and S.
marcescens were completely absent in LF, HRM,
, and HRC, EBM and DW. The higher level of isolation of E. coli and S. dysentery was found in CMS
compared to the EPA, while the higher isolation rate Samonella
sp. K. pneumonia and Enterobacter sp. were seen
in EBC than PBC. On the other hand, the higher the percentage distribution
E. coli, S. dysentery,
Samonella sp. observed in PBM compared with
EBM, while the higher isolation rate of Enterobacter
sp. was found in EBM, than PBM. Antibacterial activity: in vitro antibacterial activity >> << different extracts of leaves of M. oleifera against >> << engaged bacteria qualitatively assess the presence or absence of
braking zones. On the basis of
shown in Table 4, methanol, ethyl acetate, chloroform
and hexane extracts of M. oleifera letter
showed promising antibacterial activity against all strains of Escherichia >> << of bacteria. Methanol extract
exhibited great potential antibacterial activity against all tested >> << bacteria such as E. coli, S. dysentery, salmonellosis
Spain, Enterobacter sp. K. pneumonia, S. marcescens with corresponding zones of inhibition
21. 2, 19. 1, 23. 2, 19. 5, 18. 4 and 17. 0 mm. Ethyl acetate >> << extract also showed strong antibacterial effect against all >> << strains of Escherichia their inhibition zone from

13. 2 and 20. 2 mm. Chloroform
extract has a moderate antibacterial activity against all strains
Escherichia (inhibition zones. 12. 1 to 15 May
mm), while the hexane extract was moderate and low >> ; << antibacterial effect (inhibition zones: 8 0 14 5 mm.) .. The activity of extracts compared with standard antibiotic tetracycline
. In this study, in all cases, methanol and ethyl
acetate extract exhibited higher antibacterial activity compared
refer to the antibiotic tetracycline. Blinds do not inhibit growth of each
bacteria tested. The minimum inhibitory concentration (MIC): As shown in Table
5, methanol extract of M. oleifera letter found
more susceptible to all investigated bacteria like E. coli, S. dysentery, Salmonella sp. , Enterobacter sp. K. pneumonia, S. marcescens with MIC values from
62. From 5 to 250 g / ml. MIC values
ethyl acetate extract against employees isolates
bacterium Escherichia coli have been reported in the range of 62. 5 -
500 g / ml. On the other hand, chloroform and hexane
extract showed that inhibits the activity against all tested >> << bacteria as MIC in the range from 250 to 1000 g / ml
. In this study, methanol extract showed high
antibacterial activity against tested isolates >> << compared to other extracts MIC values. DISCUSSION
In this study, the presence of E. coli bacteria in samples collected
indication of bacterial contamination >> << as a potential source of diarrheal pathogens.
Frequency of diarrhea as a result of the epidemic
with intestinal bacterial transmission. Percentage distribution >> << intestinal bacteria show that E. Table-4: Antibacterial activity of Moringa oleifera L. leaves and extract juice on some bacteria E. coli. Bacteria Fresh juice of leaves aqueous extract ethanol extract would be a positive control
Juicea powder from fresh leaves of dried leaves of fresh leaves dried Tetracycline
fresh leaves juiceb Cold Hot Cold Hot leaves (30 g CD-1)
E. coli 17. 70. 2 40. 450. 37 16. 8 0. 12-th-th-th 210. 16 and 19. 030. 44
dysentery S. 23. 10. 08 35. 40. August 14. 92. 20-th-th-th 190. 48 and 17. 02. 24
Salmonella sp. 18. 00. 04 37. 550. 44 8. 00. 20-th-th-th 230. 40 and 22. 50. 04
Enterobacter sp. 18. 00. 04 39. 250. 2 14. 50. 40-th-th-th 190. 24 and 19. 161. 02
K. pneumonia 19. 42. 16 35. 150. 12 11. 860. 04-th-th-th 180. 25 and 12. 50. 20
S. marcescens 18. 00. 04 35. 150. 12 11. 00. 40-th-th-th 170. 12 and 11. 161. The value of 02
presented as mean southeast of triplicate experiments. I: no detection, the diameter of inhibition zone including disc diameter of 6
mm (tested volume of 10 l Disk-1), b Diameter of inhibition zone including disc diameter of 6 mm (tested volume of 1000 Disc-1)
Nepal Medical College journal.