Frequency of diarrhea as a result of the epidemic

16

Samples were considered including hand-washing children

(part breastfeeding and children exclusively breastfed

children) and their mothers (partially breastfed, mother

exclusively breastfeeding mother), food dishes, the remnants

food and drinking water. The overall level of isolation of Escherichia coli, S. dysentery, Salmonella sp. , Enterobacter sp. ,

K. pneumonia, and Serratia marcescens from the collected samples

turned 38. 01, 23. 64, 18. 84, 9. 26, 6. 70 >> << and strattera 3. 51%, respectively (Table 3). The highest isolation rate

E. coli, S. dysentery and Samonella sp. ,

Enterobacter sp. K. pneumonia, S. marcescens

were seen in the remains of a meal, PBM, EBC and their mother

EBC, and feeding dishes, respectively. In >> << On the other hand, the low rate of selection of Escherichia coli, S. dysentery

, and Samonella sp. observed in FA, DW, LF,

, respectively, while the Enterobacter sp. K. pneumonia and S.

marcescens were completely absent in LF, HRM,

, and HRC, EBM and DW. The higher level of isolation of E. coli and S. dysentery was found in CMS

compared to the EPA, while the higher isolation rate Samonella

sp. K. pneumonia and Enterobacter sp. were seen

in EBC than PBC. On the other hand, the higher the percentage distribution

E. coli, S. dysentery,

Samonella sp. observed in PBM compared with

EBM, while the higher isolation rate of Enterobacter

sp. was found in EBM, than PBM. Antibacterial activity: in vitro antibacterial activity >> << different extracts of leaves of M. oleifera against >> << engaged bacteria qualitatively assess the presence or absence of

braking zones. On the basis of

shown in Table 4, methanol, ethyl acetate, chloroform

and hexane extracts of M. oleifera letter

showed promising antibacterial activity against all strains of Escherichia >> << of bacteria. Methanol extract

exhibited great potential antibacterial activity against all tested >> << bacteria such as E. coli, S. dysentery, salmonellosis

Spain, Enterobacter sp. K. pneumonia, S. marcescens with corresponding zones of inhibition

21. 2, 19. 1, 23. 2, 19. 5, 18. 4 and 17. 0 mm. Ethyl acetate >> << extract also showed strong antibacterial effect against all >> << strains of Escherichia their inhibition zone from

13. 2 and 20. 2 mm. Chloroform

extract has a moderate antibacterial activity against all strains

Escherichia (inhibition zones. 12. 1 to 15 May

mm), while the hexane extract was moderate and low >> ; << antibacterial effect (inhibition zones: 8 0 14 5 mm.) .. The activity of extracts compared with standard antibiotic tetracycline

. In this study, in all cases, methanol and ethyl

acetate extract exhibited higher antibacterial activity compared

refer to the antibiotic tetracycline. Blinds do not inhibit growth of each

bacteria tested. The minimum inhibitory concentration (MIC): As shown in Table

5, methanol extract of M. oleifera letter found

more susceptible to all investigated bacteria like E. coli, S. dysentery, Salmonella sp. , Enterobacter sp. K. pneumonia, S. marcescens with MIC values ​​from

62. From 5 to 250 g / ml. MIC values ​​

ethyl acetate extract against employees isolates

bacterium Escherichia coli have been reported in the range of 62. 5 -

500 g / ml. On the other hand, chloroform and hexane

extract showed that inhibits the activity against all tested >> << bacteria as MIC in the range from 250 to 1000 g / ml

. In this study, methanol extract showed high

antibacterial activity against tested isolates >> << compared to other extracts MIC values. DISCUSSION

In this study, the presence of E. coli bacteria in samples collected

indication of bacterial contamination >> << as a potential source of diarrheal pathogens.

Frequency of diarrhea as a result of the epidemic

with intestinal bacterial transmission. Percentage distribution >> << intestinal bacteria show that E. Table-4: Antibacterial activity of Moringa oleifera L. leaves and extract juice on some bacteria E. coli. Bacteria Fresh juice of leaves aqueous extract ethanol extract would be a positive control

Juicea powder from fresh leaves of dried leaves of fresh leaves dried Tetracycline

fresh leaves juiceb Cold Hot Cold Hot leaves (30 g CD-1)

E. coli 17. 70. 2 40. 450. 37 16. 8 0. 12-th-th-th 210. 16 and 19. 030. 44

dysentery S. 23. 10. 08 35. 40. August 14. 92. 20-th-th-th 190. 48 and 17. 02. 24

Salmonella sp. 18. 00. 04 37. 550. 44 8. 00. 20-th-th-th 230. 40 and 22. 50. 04

Enterobacter sp. 18. 00. 04 39. 250. 2 14. 50. 40-th-th-th 190. 24 and 19. 161. 02

K. pneumonia 19. 42. 16 35. 150. 12 11. 860. 04-th-th-th 180. 25 and 12. 50. 20

S. marcescens 18. 00. 04 35. 150. 12 11. 00. 40-th-th-th 170. 12 and 11. 161. The value of 02

presented as mean southeast of triplicate experiments. I: no detection, the diameter of inhibition zone including disc diameter of 6

mm (tested volume of 10 l Disk-1), b Diameter of inhibition zone including disc diameter of 6 mm (tested volume of 1000 Disc-1)

Nepal Medical College journal.